NCN

Preludium – project finished

The aim of the project was to design and examine the silicon-gold matrices and carbon-polymer composites properties with specific surface morphology (micro/nanopatterns) for their biomedical applications. Research hypothesis assumed that the obtained composites are optimal biocompatible scaffolds for culture and direct growth of neural cell lines and human neural stem cells (hNSCs).

The motivation for undertaking the research is constant necessity of searching for cheap and biocompatible materials which can solve some issues concerning regenerative medicine. One of the specific challenges of tissue engineering is to obtain suitable scaffolds for the nervous system regeneration. Traumatic insults of central nervous system such as traumatic brain injury and spinal cord injury often effect the sensory and motor function disorders. Furthermore, the consequence of many neurodegenerative complaints such as Parkinson’s, Alzheimer’s and Huntington’s disease is neuronal declining. Central nervous system has very limited capacity to regeneration in contrast peripheral nervous system can regenerate. In case of the nervous system regeneration, scaffolds should exhibit biocompatibility, immune inertness, relevant mechanical properties and surface topography. The advantage is conductivity of the carbon-polymer biomaterials. Particularly interesting seems to be connection of carbon nanomaterials (GR and CNT) with polymeric materials. These materials have high electrical potential which can be used in the regeneration of nervous system and have a high scientific potential.
 
For composites fabrication biocompatible polymers such as collagen which naturally occurs in the body, biodegradable PCL, non-biodegradable PDMS and having conductive properties polypyrole were selected. Two types of carbon nanomaterials were chosen i.e. carbon nanotubes (CNT) and graphene (GR). Production of nanopatterns on obtained composites surfaces can improve the cell adhesion and stimulate them for targeted growth. Research tasks were be performed entirely at NanoBioMedical Centre Adam Mickiewicz University in Poznan. All matrices were characterized by means of scanning electron microscopy (SEM) and atomic force microscopy (AFM). In investigation stem cells (Neural Stem Cells) for growth, arrangement and cell adhesion studies were be used.

Publications:
  1. Milestones and current achievements in development of multifunctional bioscaffolds for medical application; Litowczenko, MJ. Woźniak-Budych, K. Staszak, K. Wieszczycka, S. Jurga, B. Tylkowski doi:10.1016/j.bioactmat.2021.01.007 Bioactive Materials, 2021 (8.724 IF).
  2. Unique cellular network formation guided by heterostructures based on reduced graphene oxide – Ti3C2Tx MXene hydrogels; Wychowaniec*, J. Litowczenko*, K. Tadyszak, V. Natu, C. Aparicio, B. Peplińska, M. W. Barsoum, M. Otyepka, B. Scheibe*; doi.org/10.1016/j.actbio.2020.08.010 Acta Biomaterialia, 2020 (7.24 IF) ♦These authors contributed equally to this work. *corresponding authors
  3. Groove-patterned surfaces induce morphological changes in cells of neuronal origin; J. Litowczenko♦, BM. Maciejewska♦, JK. Wychowaniec, M. Kościński, A. Goździcka-Józefiak, S. Jurga, A. Warowicka. doi: 10.1002/jbm.a.36733 Journal of Biomedical Materials Research Part A., 2019 (3.525 IF)
  4. Fabricating versatile cell supports from nano- and micro-sized graphene oxide flakes; Wychowaniec♦,
    J. Litowczenko    ♦, K. Tadyszak; doi: 10.1016/j.jmbbm.2019.103594 Journal of the Mechanical Behavior of Biomedical Materials, 2020 (3.670 IF) ♦These authors contributed equally to this work.
  5. Designing biocompatible spin-coated multiwall carbon nanotubes-polymer composite coatings; D. Maziukiewicz, BM. Maciejewska, Litowczenko, M. Kościński, A. Warowicka, JK. Wychowaniec, S. Jurga; doi: 10.1016/j.surfcoat.2019.125199; Surface and Coatings Technology, 2020 (3.784 IF)
  6. Cytotoxicity of versatile nano-micro-particles based on hierarchical flower-like ZnO; N. Babayevska, Litowczenko♦*, JK. Wychowaniec♦*, I. Iatsunskyi, M. Jarek, P. Florczak, S. Jurga; doi: 10.1016/j.apt.2019.10.03; Advanced Powder Technology, 2020 (4.217 IF) ♦These authors contributed equally to this work, *corresponding author
  7. Sucrose based cellular glassy carbon for biological applications; K. Tadyszak*, Litowczenko*, Ł. Majchrzycki, P. Jeżowski, K. Załęski, B. Scheibe; doi: 10.1016/j.matchemphys.2019.122033; Materials Chemistry and Physics 2020 (3,408 IF) *corresponding authors
  8. Biomedical Applications of Graphene-Based Structures; Tadyszak, J. Wychowaniec, J. Litowczenko; Nanomaterials,2018, 8(11), 944; doi: 10.3390/nano8110944 (4.514 IF)
Books
  • When Art Meets Science NanoBioMedical Centre at AMU, Poland, 2019,
    DOI: 10.5281/zenodo.2533342, ISBN: 978-83-950131-1-9 K.; K. Tadyszak, O. Ivashchenko, B. Peplińska, Litowczenko, et al.
  • Microstructures photo album NanoBioMedical Centre, AMU, Poland, 2018,
    DOI: 10.5281/zenodo.2533061, ISBN: 978-83-950131-0-2.     Tadyszak, O. Ivashchenko, B. Peplińska, J. Litowczenko, et al.
Oral presentations
  1. Spatial topological confinement dictates the effective growth and differentiation of neural stem cells Litowczenko-Cybulska, BM Maciejewska, J. Wychowaniec; 31st Annual Conference of the European Society for Biomaterials (ESB 2021) and 43rd Annual Congress of the Iberian Society for Biomechanics and Biomaterials (SIBB); 5-9.09.2021 Porto, Portugal (virtual) – wygłoszenie krótkiego referatu
  2. Tuning responsivity of cellular behavior via topographical cues Litowczenko, B. M. Maciejewska, J. K. Wychowaniec; Design and Function of Responsive Nanocomposites, 20-21.05.2021, Dublin, Ireland (virtual) – wygłoszenie referatu
  3. Patterned guiding cues for differentiation of neuronal cells; J Litowczenko, BM. Maciejewska,
    Wychowaniec, M. Kościńśki, A. Goździcka-Józefiak, S. Jurga; Functional Surfaces: How Surfaces Control Our World; SCI The Society of Chemical Industry 12.06.2019; 14/15 Belgrave Square, London, United     Kingdom.
  4. The influence of (nano)materials topography on the behavior and differentiation of human neural cells; Litowczenko, BM. Maciejewska, A. Warowicka, J.K. Wychowaniec, M. Kościński, A.Goździcka-Józefiak, S. Jurga; NanoTech Poland 2019 & Nanotechnology and Innivation in the Baltic Sea Region, 5-8.06.2019, Poznań, Poland.
  5. Patterned matrices for direct growth of neuronal cells – 5th Kiel Days in Poznań;
    Litowczenko;     Joint Symposium on Nanomaterials and Their Application in Materials Science and Biomedicine, 8.11.2018, Poznań, Poland.
  6. Nanopatterned matrices for direct growth of neural cells; Litowczenko, A. Warowicka,
    BM. Maciejewska, J. Wychowaniec, M. Kościński, K. Załęski, I. Iatsunskyi, A. Goździcka-Józefiak, S. Jurga; NanoTech 2018 Poland International Conference & Exhibition, 6-9.06.2018, Poznań, Poland.
  7. Unique cellular network formation guided by heterostructures based on reduced graphene oxide – Ti3C2Tx MXene hydrogels, JK. Wychowaniec, Litowczenko, K. Tadyszak, V. Natu, C. Aparicio, B. Peplińska, MW. Barsoum, M. Otyepka, B Scheibe; Final Multicomp Meeting 2020 Slovenj Gradec, Slovenia; 24-25.09.2020
  8. VI Warsztaty Naukowe Instytutu Biologii Eksperymentalnej Uniwersytetu im. Adama Mickiewicza w Poznaniu. „Matryce o określonej topografii powierzchni dla wzrostu ludzkich komórek neuralnych”; Litowczenko, A. Warowicka, B M. Maciejewska, J. Wychowaniec, M. Kościński, I. Iatsunskyi, A. Goździcka-Józefiak, S. Jurga; 15.06.2018
  9. Nanopatterned matrices for tissue engineering MWCNT based materials as potential scaffolds for adhesion and growth of cells; Litowczenko, A. Warowicka, B. Maciejewska, M. Kościński, K. Załęski, I. Iatsunskyi and S. Jurga; NanoTech 2017 Poland International Conference & Exhibition, 1-3.06.2017, Faculty of Physics, Adam Mickiewicz University in Poznań.
  10. Matryce o określonej topografii powierzchni dla wzrostu i różnicowania neuralnych komórek macierzystych; Litowczenko, B M. Maciejewska, A Warowicka. J K Wychowaniec, M. Kościnski, A. Goździcka-Józefiak., S. Jurga; IX Krajowa konferencja NanoTechnologii 1-3 lipca 2019, Wrocław
  11. Designing and creating scaffolds for culturing of neural stem cells; Litowczenko, Three Minute Thesis®, University of Economics, 1-3.03.2017 Poznan
Posters
  1. Carbon-polymer porous thin film sprepared by spin-coated method; J. Litowczenko, B. Maciejewska, A. Warowicka, M. Kościński, S. Jurga; NanoFlorida 2017, 23-24.08.2017, Florida, USA
  2. Nanopatterned matrices for direct growth of neural cells; Litowczenko, A. Warowicka, B. M. Maciejewska, J. Wychowaniec, B. Wereszczyńska, M. Kościński, A. Goździcka-Józefiak, S. Jurga; Ampere NMR School, 10-16.06.2018, Zakopane, Poland.
  3. Fine-tuning neural stem cells – 2.5D multiwall carbon nanotubes surfaces interactions for engineering functional regenerative scaffolds; Litowczenko, B. M. Maciejewska, A. Warowicka, J. Wychowaniec, M. Kościński, S. Jurga; NanoTech 2018 Poland International Conference & Exhibition, 6-9.06.2018, Poznan, Poland.
  4. The fabrication and cytotoxicity dependence of macro-sized scaffolds made of nano- and micro-sized graphene oxide flakes, J. Litowczenko, JK Wychowaniec, K Tadyszak, I Poznańskie Mikrosympozjum Grafenowe, Centrum Zaawansowanych Technologii UAM w Poznaniu, 2019-03-15